Differentiation of Retinal Glial Cells From Human Embryonic Stem Cells by Promoting the Notch Signaling Pathway

SH Chung; W Shen; KC Davidson; A Pébay; RCB Wong; B Yau; M Gillies

Journal article

Differentiation of Retinal Glial Cells From Human Embryonic Stem Cells by Promoting the Notch Signaling Pathway

SH Chung, W Shen, KC Davidson, A Pébay, RCB Wong, B Yau, M Gillies

Frontiers in Cellular Neuroscience | FRONTIERS MEDIA SA | Published : 2019

DOI: 10.3389/fncel.2019.00527

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Abstract

Dysfunction of retinal glial cells, particularly Müller cells, has been implicated in several retinal diseases. Despite their important contribution to retinal homeostasis, a specific way to differentiate retinal glial cells from human pluripotent stem cells has not yet been described. Here, we report a method to differentiate retinal glial cells from human embryonic stem cells (hESCs) through promoting the Notch signaling pathway. We first generated retinal progenitor cells (RPCs) from hESCs then promoted the Notch signaling pathway using Notch ligands, including Delta-like ligand 4 and Jagged-1. We validated glial cell differentiation with qRT-PCR, immunocytochemistry, western blots and fl..

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University of Melbourne Researchers

Alice Pebay Author

Raymond Wong Author

Kathryn Davidson Author

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Grants

Awarded by Australian Research Council

Funding Acknowledgements

This project was supported by grants from the Ophthalmic Research Institute of Australia, Commercial Development and Industry Partnerships at the University of Sydney and the Lowy Medical Research Institute Pty Ltd. AP was supported by a Australian Research Council Future Fellowship (FT140100047) and a National Health and Medical Research Council (NHMRC) Senior Research Fellowship (1154389).